With cryo-ET we can obtain the 3D reconstruction of objects that exist as unique copies, either in isolation or within cells. In many cases, however, the questions we are interested in require a higher and more isotropic resolution. In these cases we can average a set of structures obtained by cryo-ET, in isolation or within intact cells, and also solve the structure of the isolated complex at higher resolution by single particle methods. RNPs, bacteriophages, and complex RNA systems are examples of problems that we should tackle with a combination of techniques.