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Bacterial Cell Surfaces and Interactions

MOVIE: Surface of an intact Lysinibacillus sphaericus cell: S-layer(MOV 2.4M)
3D architecture of the intact S-layer (wt SbpA) covering the surface of Lysinibacillus sphaericus by cryo-ET.
Slices from a cryo-ET reconstruction of self-assembled S-layer films in solution (scale bar 50 nm).
Cryo-ET reconstruction of in-vitro self-assembled rSbpA S-Layer.
Macromolecular architecture -cryo-ET subtomographic averaged lattice structure of the monolayer S-layer.
rSbpA S-layer: C4-symmetric homotetrameric lattice subunit.
S-Layer bilayer in solution top view. Cryo-ET model representation: colored spheres are subunit locations. S-Layer bilayer in solution side viewu. Cryo-ET model representation: colored spheres are subunit locations.
Cryo-ET slices through reconstructions of S-layer bilayer lattices - False colors. Isosurface rendering of a 3D reconstructed patch at the center.
MOVIE: 3D reconstruction of a Mariprofundus ferroxydans cell - a volume-rendered reconstruction. Blue is high contrast.(MPEG 3.3M)
Mariprofundus ferroxydans(PV1): a volume-rendered 3D-reconstruction. Blue is high contrast. Mariprofundus ferroxydans(PV1): a volume-rendered 3D-reconstruction. Orange is high contrast-Fe.
Mariprofundus_ferroxydans(PV1). CryoET_Slice_and_isosurface.Red_is_high_contrast-Fe. Morphology of PV1. Segmented IM and isosurface rendering of the iron-oxyhydroxide stalk.
Intact Ultrastructure of Mariprofundus ferroxydans stalk. (A) Isosurface rendering of a stalk at the cell wall origin. Color code: red to blue from right-most to left-most side. (B) A 150 voxels-thick 3D section from a volume-rendered tomographic reconstruction of a stalk. (C) and (D): Cryo-ET orthogonal slices through stalk in (B). Scales: (B), (C): 250 nm; (D): 100 nm.
Cryo-ET_2D-projection of part of a stalk and HR-cryo-TEM of a small area near the outside boundary. (A) scale bar 500 nm. (B) 120Kx image 450 nm by side (scale 100 nm). A magnified view from a small region on a particle, within the blue box, is shown in the top-left inset. The plot profile to the right of the inset was computed along the blue line in the inset; the distance between peaks, corresponding to the lattice plane spacing, is ~6.6 Å. Inset scale bar 25 Å. Yellow arrows point to "blade-shaped" particles.
HR-cryo-TEM 2D projections of a region of a stalk. (A) A 80 Kx 2D projection of a region of a stalk. The image is ~400 nm by side, scale bar 100 nm. (B) A 200 Kx 2D projection of a central region of a stalk. The image is ~245 nm by side, scale bar 50 nm. The plot profile at the bottom of the inset was computed along the blue line in the inset; The lattice plane spacing is ~5.5 Å Yellow arrows point to similar "blade-shaped" particles.

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